“Thermofisher Lunch seminar” - Tuesday, July 4th 2017, 1:00 - 2:00 pm
Speeding up Biomarker Discovery: Advanced Translational Proteomics Workflow with High Resolution Accurate Mass (HRAM) MS
Claire Dauly, Omics Sales Support Manager Europe
Diseases like cancer are complex and require another level of discipline in analytical proteomics measurements. Having set the standards for discovery research with deep comprehensive proteome profiles, new advances in Orbitrap technology and workflows allow for large scale analysis across large sample numbers.
Here we present a highly robust novel capillary-flow LC-MS platform that combines a capillary-flow chromatographic system (capLC), a new 150 µm column and a new Thermo Scientific™ Q Exactive™ HF-X mass spectrometer. The Q Exactive HF-X encompasses a high capacity transfer tube (HCTT), an electrodynamic ion funnel for increased ion flux and a scan rate of 40 Hz.
The new capLC-MS platform performances were assessed for shotgun and targeted high-resolution accurate-mass (HRAM) proteomics experiments demonstrating high sensitivity and robustness for the analysis of complex samples including bio-fluids.
A novel high resolution MS1 based data independent acquisition (DIA) method was also developed for comprehensive proteome profiling. The capLC-MS platform identifies and quantifies more than 4800 protein groups and 46000 peptide precursors in a 1-hour total run time (1% FDR and median CV<10%) delivering the analytical reproducibility and scalability required for rigorous results that matter.
To participate in the lunch seminar please contact :